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KMID : 0613820080180070907
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Journal of Life Science
2008 Volume.18 No. 7 p.907 ~ p.911
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Determination of Seed Purity in Radish (Raphanus sativus L.) Using Allozyme
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Huh Man-Kyu
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Abstract
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Radish (Raphanus sativus L.) is one of very important crop plants in the world. It is very important to determine hybrid seed quality in the production of hybrid Brassica vegetable seeds to avoid unacceptable contamination with self-inbred (sib) seeds. The allozyme for evaluating seed purity in a commercial F1-hybrid radish cultivar is demonstrated. Three hundred sixty seeds from the male and female harvest were subsequently screened for seed purity using 27 isozyme loci. Especially, F1 hybrids of radish, Per-1 (aa x bb), Lap-1 (aa x bb), Est-1 (aa x bb) were presented clear hybrid bands. Est-1 locus revealed that 15 (8.3%) seeds from the female harvest and 26 (14.4%) seeds from the male harvest were sibs. It maintains higher than average level of genetic diversity compared with their correspondent parents. Shannon’s index of phenotypic diversity (I) of hybrids was the highest of all accessions (R. sativus L. cv. Daepeng, R. sativus L. cv. Backza, and their hybrids). The allozyme may lead to a better insight into the hybrid seed purity.
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KEYWORD
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Allozyme, radish, Raphanussativus, seedpurity
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